Camphora longepaniculata is an important economic crop renowned for its rich volatile terpene compounds.Terpene synthases (TPS) are key enzymes in the biosynthesis of these compounds, playing significant Garters roles in plant growth, development, and secondary metabolism.In this study, a total of 86 TPS genes were identified in Camphora longepaniculata, which were classified into five groups based on their evolutionary relationships.Analysis of cis-regulatory elements revealed associations between TPS genes and processes related to plant growth, development, and environmental stress responses.Gene Ontology (GO) enrichment analysis indicated that these TPS genes are predominantly linked to various enzymatic activities.
Furthermore, analysis of duplication events revealed that tandem duplications (TD) and whole genome duplications (WGD) are major driving forces in the evolution of the TPS gene family.Notably, 18 TPS genes were found to Horns be upregulated in high essential oil content varieties of Camphora longepaniculata.RT-qPCR validation further confirmed that TPS26, TPS28, and TPS47 exhibit upregulated expression during leaf development, highlighting their potential involvement in terpene biosynthesis during this crucial developmental stage.These findings lay a solid foundation for further exploration of the functions of TPS genes in Camphora longepaniculata.